Lino Gonzalez
Scientist, Protein Chemistry-
My Keywords
Protein Chemistry, Immunology -
My Department
Protein Chemistry
I joined Genentech in 2001 for the simple reason that it is one of the best places in the world to do science. The scientific resources, infrastructure and people are unparalleled. This creates a fertile ground for quickly moving a project from an idea to a reality. It was also clear to me that this was a great place to do science by the number of scientists that had spent a majority of their careers working here.
My Focus
My lab focuses on identifying novel receptor-ligand or co-receptor interactions. Now that the human genome is complete, one of the next steps is to understand the interactions between all transmembrane and secreted proteins, which represent about one-third of all human genes. This knowledge would greatly aid in characterizing the biological function of many receptors/ligand pairs and their potential as therapeutic targets. We concentrate on receptors of the immune system, in particular the immunoglobulin receptor family.
Surprisingly, a significant number of these receptors still remain orphans. In order to identify candidate partners, we have utilized Genentech's SPDI (Secreted Protein Discovery Initiative) protein library, consisting of over 1,000 purified, secreted proteins. Currently, we are using surface plasmon resonance (SPR) and Bio-layer interferometry (BLI) technology to screen hundreds of interactions per day. To date, this work has led to the identification of several novel interactions that are now being evaluated for their therapeutic potential.
Publications & Recognition
- The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells
- Nat Immunol 2009 Jan; 10(1): 48-57. Epub 2008 Nov 16
- Yu X, Harden K, Gonzalez LC, Francesco M, Chiang E, Irving B, Tom I, Ivelja S, Refino CJ, Clark H, Eaton D, Grogan JL
- View Abstract on PubMed
- Attenuating lymphocyte activity: the crystal structure of the BTLA-HVEM complex
- J Biol Chem 2005 Nov 25; 280(47): 39553-61
- Compaan DM, Gonzalez LC, Tom I, Loyet KM, Eaton D, Hymowitz SG
- View Abstract on PubMed
- A coreceptor interaction between the CD28 and TNF receptor family members B and T lymphocyte attenuator and herpesvirus entry mediator
- Proc Natl Acad Sci U S A 2005 Jan 25; 102(4): 1116-21
- Gonzalez LC, Loyet KM, Calemine-Fenaux J, Chauhan V, Wranik B, Ouyang W, Eaton DL
- View Abstract on PubMed
- Stanford University, California
- Postdoctoral Fellow
- 1998-2001
- Yale University
- Postdoctoral Fellow
- 1997
- University of California, Berkeley, Department of Molecular and Cell Biology
- Ph.D
- 1991-1996
- University of California, Santa Barbara, Department of Chemistry
- B.S
- 1987-1991
Publications & Recognition
- The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells
- Nat Immunol 2009 Jan; 10(1): 48-57. Epub 2008 Nov 16
- Yu X, Harden K, Gonzalez LC, Francesco M, Chiang E, Irving B, Tom I, Ivelja S, Refino CJ, Clark H, Eaton D, Grogan JL
- View Abstract on PubMed
- Attenuating lymphocyte activity: the crystal structure of the BTLA-HVEM complex
- J Biol Chem 2005 Nov 25; 280(47): 39553-61
- Compaan DM, Gonzalez LC, Tom I, Loyet KM, Eaton D, Hymowitz SG
- View Abstract on PubMed
- A coreceptor interaction between the CD28 and TNF receptor family members B and T lymphocyte attenuator and herpesvirus entry mediator
- Proc Natl Acad Sci U S A 2005 Jan 25; 102(4): 1116-21
- Gonzalez LC, Loyet KM, Calemine-Fenaux J, Chauhan V, Wranik B, Ouyang W, Eaton DL
- View Abstract on PubMed
- Crystal structure of the N-terminal domain of syntaxin 6
- Manuscript in preparation 2001.
- Misura K, Jason Bock J, Gonzalez LC Jr, Scheller RH, Weis WI
- View Abstract on PubMed
- Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and Syntaxin 1a
- J Biol Chem 2001 Nov 2; 276(44): 41301-9
- Misura KM, Gonzalez LC Jr, May AP, Scheller RH, Weis WI
- View Abstract on PubMed
- A novel snare N-terminal domain revealed by the crystal structure of Sec22b
- J Biol Chem 2001 Jun 29; 276(26): 24203-11
- Gonzalez LC Jr, Weis WI, Scheller RH
- View Abstract on PubMed
- nSec1 binds a closed conformation of syntaxin1A
- J Cell Biol 2000 Jan 24; 148(2): 247-52
- Yang B, Steegmaier M, Gonzalez LC Jr, Scheller RH
- View Abstract on PubMed
- Regulation of membrane trafficking: structural insights from a Rab/effector complex
- Cell 1999 Mar 19; 96(6): 755-8
- Gonzalez L Jr, Scheller RH
- View Abstract on PubMed
- SNARE interactions are not selective. Implications for membrane fusion specificity
- J Biol Chem 1999 Feb 26; 274(9): 5649-53
- Yang B, Gonzalez L Jr, Prekeris R, Steegmaier M, Advani RJ, Scheller RH
- View Abstract on PubMed
- Crystal structures of a single coiled-coil peptide in two oligomeric states reveal the basis for structural polymorphism
- Nat Struct Biol 1996 Dec; 3(12): 1002-9
- Gonzalez L Jr, Brown RA, Richardson D, Alber T
- View Abstract on PubMed
- Buried polar residues and structural specificity in the GCN4 leucine zipper
- Nat Struct Biol 1996 Dec; 3(12): 1011-8
- Gonzalez L Jr, Woolfson DN, Alber T
- View Abstract on PubMed
- An engineered allosteric switch in leucine-zipper oligomerization
- Nat Struct Biol 1996 Jun; 3(6): 510-5
- Gonzalez L Jr, Plecs JJ, Alber T
- View Abstract on PubMed
Awards & Honors
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Howard Hughes Medical Institute Postdoctoral Research Fellow - 2000-2001
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National Science Foundation Minority Postdoctoral Research Fellow - 1998-1999
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Howard Hughes Medical Institute Postdoctoral Research Fellow - 1997
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Patricia Robert Harris Fellowship for Graduate Studies - 1991-1994
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Summer Research Fellowship, University of California, Santa Cruz - 1991
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Undergraduate Research Grant Award, University of California, Santa Barbara - 1990
