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Scientist Profile

Richard Carano

Senior Scientist
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"Working at Genentech has offered me the rewarding opportunity to not only contribute to the development of new imaging diagnostics, but also to contribute in the development of successful treatments for diseases."
 

I joined Genentech's Biomedical Imaging Group in 2000. My educational background is in biomedical and electrical engineering with a focus on medical imaging and image analysis. Prior to joining Genentech, I had worked in both academia and industry developing imaging methods to study disease and monitor therapy. I chose to come to Genentech because it offers the exciting opportunity to develop novel imaging methods in tandem with the development of novel therapeutics, where imaging can be used not only to assess the efficacy of the compound, but also to provide insight into the mode of action of the drug.

Genentech provides the resources and the collaborative environment needed to tackle such tasks in an efficient and thorough manner. Since joining Genentech, my research efforts have been directed toward the development of micro-computed tomography, MRI, and ultrasound imaging techniques to quantify structural and physiological parameters. Our research efforts focus on developing the appropriate imaging and analysis methodologies that will best answer the biological question we are addressing.

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My Focus

We have been developing a suite of imaging methods and analysis techniques to quantify tumor angiogenesis. These endpoints include micro-ct angiographic techniques to quantify vessel structures and dynamic imaging techniques (MRI and Ultrasound) for studying tumor permeability and perfusion. We also have been working on multispectral MRI methods to identify viable tumor tissue and quantify the pathophysiology of the viable tumor.

The current research focus of our lab includes several areas. We have been developing a suite of imaging methods and analysis techniques to quantify tumor angiogenesis. These endpoints include micro-ct angiographic techniques to quantify vessel structures and dynamic imaging techniques (MRI and Ultrasound) for studying tumor permeability and perfusion. We also have been working on multispectral MRI methods to identify viable tumor tissue and quantify the pathophysiology of the viable tumor. These methods attempt to address the high degree of structural and physiological heterogeneity seen within solid tumors. Other areas of research include employing imaging to quantify bone loss in models of rheumatoid arthritis and inflammation in models of multiple sclerosis.

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Publications & Recognition
  • Vessel imaging with viable tumor analysis for quantification of tumor angiogenesis.
  • Magn Reson Med. 2010 Jun;63(6):1637-47.
  • Ungersma SE, Pacheco G, Ho C, Yee SF, Ross J, van Bruggen N, Peale FV Jr, Ross S, Carano RA.
  • View Abstract on PubMed
  • Viable tumor tissue detection in murine metastatic breast cancer by whole-body MRI and multispectral analysis.
  • Magn Reson Med. 2009 Dec;62(6):1423-30.
  • Barck KH, Willis B, Ross J, French DM, Filvaroff EH, Carano RA.
  • View Abstract on PubMed
  • Quantifying antivascular effects of monoclonal antibodies to vascular endothelial growth factor: insights from imaging.
  • Clin Cancer Res. 2009 Nov 1;15(21):6674-82. Epub 2009 Oct 27.
  • O'Connor JP, Carano RA, Clamp AR, Ross J, Ho CC, Jackson A, Parker GJ, Rose CJ, Peale FV, Friesenhahn M, Mitchell CL, Watson Y, Roberts C, Hope L, Cheung S, Reslan HB, Go MA, Pacheco GJ, Wu X, Cao TC, Ross S, Buonaccorsi GA, Davies K, Hasan J, Thornton P, del Puerto O, Ferrara N, van Bruggen N, Jayson GC.
  • View Abstract on PubMed
 
View All Publications & Recognition
 
  • Worcester Polytechnic Institute
  • Biomedical Engineering, Ph.D.
  • 1998
  • University of California, Davis
  • Electrical Engineering and Computer Science, M.S.
  • 1985
  • Rutgers University
  • Electrical Engineering, B.S.
  • 1981
Publications & Recognition
  • Vessel imaging with viable tumor analysis for quantification of tumor angiogenesis.
  • Magn Reson Med. 2010 Jun;63(6):1637-47.
  • Ungersma SE, Pacheco G, Ho C, Yee SF, Ross J, van Bruggen N, Peale FV Jr, Ross S, Carano RA.
  • View Abstract on PubMed
  • Viable tumor tissue detection in murine metastatic breast cancer by whole-body MRI and multispectral analysis.
  • Magn Reson Med. 2009 Dec;62(6):1423-30.
  • Barck KH, Willis B, Ross J, French DM, Filvaroff EH, Carano RA.
  • View Abstract on PubMed
  • Quantifying antivascular effects of monoclonal antibodies to vascular endothelial growth factor: insights from imaging.
  • Clin Cancer Res. 2009 Nov 1;15(21):6674-82. Epub 2009 Oct 27.
  • O'Connor JP, Carano RA, Clamp AR, Ross J, Ho CC, Jackson A, Parker GJ, Rose CJ, Peale FV, Friesenhahn M, Mitchell CL, Watson Y, Roberts C, Hope L, Cheung S, Reslan HB, Go MA, Pacheco GJ, Wu X, Cao TC, Ross S, Buonaccorsi GA, Davies K, Hasan J, Thornton P, del Puerto O, Ferrara N, van Bruggen N, Jayson GC.
  • View Abstract on PubMed
  • USP1 deubiquitinates ID proteins to preserve a mesenchymal stem cell program in osteosarcoma.
  • Cell. 2011 Sep 16;146(6):918-30.
  • Williams SA, Maecker HL, French DM, Liu J, Gregg A, Silverstein LB, Cao TC, Carano RA, Dixit VM.
  • View Abstract on PubMed
  • Global defects in collagen secretion in a Mia3/TANGO1 knockout mouse.
  • J Cell Biol. 2011 May 30;193(5):935-51
  • Wilson DG, Phamluong K, Li L, Sun M, Cao TC, Liu PS, Modrusan Z, Sandoval WN, Rangell L, Carano RA, Peterson AS, Solloway MJ.
  • View Abstract on PubMed
  • Specific Btk inhibition suppresses B cell- and myeloid cell-mediated arthritis.
  • Nat Chem Biol. 2011 Jan;7(1):41-50.
  • Di Paolo JA, Huang T, Balazs M, Barbosa J, Barck KH, Bravo BJ, Carano RA, Darrow J, Davies DR, DeForge LE, Diehl L, Ferrando R, Gallion SL, Giannetti AM, Gribling P, Hurez V, Hymowitz SG, Jones R, Kropf JE, Lee WP, Maciejewski PM, Mitchell SA, Rong H, Staker BL, Whitney JA, Yeh S, Young WB, Yu C, Zhang J, Reif K, Currie KS.
  • View Abstract on PubMed
  • Granulocyte-colony stimulating factor promotes lung metastasis through mobilization of Ly6G+Ly6C+ granulocytes.
  • Proc Natl Acad Sci U S A. 2010 Nov 16.
  • Kowanetz M, Wu X, Lee J, Tan M, Hagenbeek T, Qu X, Yu L, Ross J, Korsisaari N, Cao T, Bou-Reslan H, Kallop D, Weimer R, Ludlam MJ, Kaminker JS, Modrusan Z, van Bruggen N, Peale FV, Carano R, Meng YG, Ferrara N.
  • View Abstract on PubMed
  • Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor.
  • Development. 2010 Nov;137(22):3753-61.
  • Seidel K, Ahn CP, Lyons D, Nee A, Ting K, Brownell I, Cao T, Carano RA, Curran T, Schober M, Fuchs E, Joyner A, Martin GR, de Sauvage FJ, Klein OD.
  • View Abstract on PubMed
  • Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies.
  • PLoS One. 2010 Sep 13;5(9).
  • Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida VI, Liu BY, Phamluong K, Cao TC, Carano RA, Ernst JA, Solloway M, Rubinfeld B, Hannoush RN, Wu Y, Polakis P, Costa M.
  • View Abstract on PubMed
  • MR angiogenesis imaging with Robo4- vs. {alpha}V{beta}3-targeted nanoparticles in a B16/F10 mouse melanoma model.
  • FASEB J. 2010 Jun 28. [Epub ahead of print]
  • Boles KS, Schmieder AH, Koch AW, Carano RA, Wu Y, Caruthers SD, Tong RK, Stawicki S, Hu G, Scott MJ, Zhang H, Reynolds BA, Wickline SA, Lanza GM.
  • View Abstract on PubMed
  • Assessing therapeutic responses in Kras mutant cancers using genetically engineered mouse models.
  • Nat Biotechnol. 2010 Jun;28(6):585-93. Epub 2010 May 23.
  • Singh M, Lima A, Molina R, Hamilton P, Clermont AC, Devasthali V, Thompson JD, Cheng JH, Bou Reslan H, Ho CC, Cao TC, Lee CV, Nannini MA, Fuh G, Carano RA, Koeppen H, Yu RX, Forrest WF, Plowman GD, Johnson L.
  • View Abstract on PubMed
  • Host-detrimental role of Esx-1-mediated inflammasome activation in mycobacterial infection.
  • PLoS Pathog. 2010 May 6;6(5).
  • Carlsson F, Kim J, Dumitru C, Barck KH, Carano RA, Sun M, Diehl L, Brown EJ.
  • View Abstract on PubMed
  • PlGF blockade does not inhibit angiogenesis during primary tumor growth.
  • Cell. 2010 Apr 2;141(1):166-77.
  • Bais C, Wu X, Yao J, Yang S, Crawford Y, McCutcheon K, Tan C, Kolumam G, Vernes JM, Eastham-Anderson J, Haughney P, Kowanetz M, Hagenbeek T, Kasman I, Reslan HB, Ross J, Van Bruggen N, Carano RA, Meng YJ, Hongo JA, Stephan JP, Shibuya M, Ferrara N.
  • View Abstract on PubMed
  • Mature dendritic cells use endocytic receptors to capture and present antigens.
  • Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4287-92. Epub 2010 Feb 8.
  • Platt CD, Ma JK, Chalouni C, Ebersold M, Bou-Reslan H, Carano RA, Mellman I, Delamarre L.
  • View Abstract on PubMed
  • Prostate-specific Klf6 inactivation impairs anterior prostate branching morphogenesis through increased activation of the Shh pathway.
  • J Biol Chem. 2009 Jul 31;284(31):21057-65. Epub 2009 Jun 3.
  • Leow CC, Wang BE, Ross J, Chan SM, Zha J, Carano RA, Frantz G, Shen MM, de Sauvage FJ, Gao WQ.
  • View Abstract on PubMed
  • Targeted depletion of lymphotoxin-alpha-expressing TH1 and TH17 cells inhibits autoimmune disease.
  • Nat Med. 2009 Jul;15(7):766-73. Epub 2009 Jun 28.
  • Chiang EY, Kolumam GA, Yu X, Francesco M, Ivelja S, Peng I, Gribling P, Shu J, Lee WP, Refino CJ, Balazs M, Paler-Martinez A, Nguyen A, Young J, Barck KH, Carano RA, Ferrando R, Diehl L, Chatterjea D, Grogan JL.
  • View Abstract on PubMed
  • Cooperation of the agonistic DR5 antibody apomab with chemotherapy to inhibit orthotopic lung tumor growth and improve survival.
  • Clin Cancer Res. 2008 Dec 1;14(23):7733-40.
  • Jin H, Yang R, Ross J, Fong S, Carano R, Totpal K, Lawrence D, Zheng Z, Koeppen H, Stern H, Schwall R, Ashkenazi A.
  • View Abstract on PubMed
  • 3D micro-CT imaging of the postmortem brain.
  • J Neurosci Methods. 2008 Jun 30;171(2):207-13. Epub 2008 Mar 27.
  • de Crespigny A, Bou-Reslan H, Nishimura MC, Phillips H, Carano RA, D'Arceuil HE.
  • View Abstract on PubMed
  • MetMAb, the one-armed 5D5 anti-c-Met antibody, inhibits orthotopic pancreatic tumor growth and improves survival.
  • Cancer Res. 2008 Jun 1;68(11):4360-8.
  • Jin H, Yang R, Zheng Z, Romero M, Ross J, Bou-Reslan H, Carano RA, Kasman I, Mai E, Young J, Zha J, Zhang Z, Ross S, Schwall R, Colbern G, Merchant M.
  • View Abstract on PubMed
  • Blocking neuropilin-2 function inhibits tumor cell metastasis.
  • Cancer Cell. 2008 Apr;13(4):331-42.
  • Caunt M, Mak J, Liang WC, Stawicki S, Pan Q, Tong RK, Kowalski J, Ho C, Reslan HB, Ross J, Berry L, Kasman I, Zlot C, Cheng Z, Le Couter J, Filvaroff EH, Plowman G, Peale F, French D, Carano R, Koch AW, Wu Y, Watts RJ, Tessier-Lavigne M, Bagri A.
  • View Abstract on PubMed
  • Quantification of viable tumor microvascular characteristics by multispectral analysis.
  • Magn Reson Med 2008 Apr 17; [Epub ahead of print]
  • Berry LR, Barck KH, Go MA, Ross J, Wu X, Williams SP, Gogineni A, Cole MJ, Van Bruggen N, Fuh G, Peale F, Ferrara N, Ross S, Schwall RH, Carano RA.
  • View Abstract on PubMed
  • Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
  • Nature 2007 Dec 6; 450(7171): 825-31.
  • Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, Van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N.
  • View Abstract on PubMed
  • Quantification of bone lesions in rheumatoid arthritis by MRI multispectral tissue class transition analysis.
  • Semin Musculoskelet Radiol 2004 Dec; 8(4): 301-11.
  • Carano RA, Lynch JA.
  • View Abstract on PubMed
  • Quantification of cortical bone loss and repair for therapeutic evaluation in collagen-induced arthritis, by micro-computed tomography and automated image analysis.
  • Arthritis Rheum 2004 Oct; 50(10): 3377-86.
  • Barck KH, Lee WP, Diehl LJ, Ross J, Gribling P, Zhang Y, Nguyen K, van Bruggen N, Hurst S, Carano RA.
  • View Abstract on PubMed
  • A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response.
  • J Biol Chem 2004 Jul 16; 279(29): 30202-9.
  • Seshasayee D, Wang H, Lee WP, Gribling P, Ross J, Van Bruggen N, Carano R, Grewal IS.
  • View Abstract on PubMed
  • Multispectral analysis of bone lesions in the hands of patients with rheumatoid arthritis.
  • Magn Reson Imaging 2004 May; 22(4): 505-14.
  • Carano RA, Lynch JA, Redei J, Ostrowitzki S, Miaux Y, Zaim S, White DL, Peterfy CG, Genant HK.
  • View Abstract on PubMed
  • Quantification of tumor tissue populations by multispectral analysis.
  • Magn Reson Med 2004 Mar; 51(3): 542-51.
  • Carano RA, Ross AL, Ross J, Williams SP, Koeppen H, Schwall RH, Van Bruggen N.
  • View Abstract on PubMed
  • Angiogenesis and bone repair.
  • Drug Discov Today 2003 Nov 1; 8(21): 980-9.
  • Carano RA, Filvaroff EH.
  • View Abstract on PubMed
  • Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover.
  • Proc Natl Acad Sci U S A 2002 Jul 23; 99(15): 9656-61.
  • Street J, Bao M, deGuzman L, Bunting S, Peale FV, Ferrara N, Steinmetz H, Hoeffel J, Cleland JL, Daugherty A, van Bruggen N, Redmond HP, Carano RA, Filvaroff EH.
  • View Abstract on PubMed
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Awards & Honors
  • Potvin Award: Outstanding Biomedical Graduate Student (WPI)
  • Tau Beta Pi, National Engineering Honor Society
  • Eta Kappa Nu, National Electrical Engineering Honor Society
 
Patents
  • Anti-ROBO4 antibodies as a tumor vasculature targeting agent for vascular imaging applications.
    R. A. D. Carano, Ryan Watts, Alexander Koch
    Nov, 2010
    US 2011/0059013 A1
  • A Phantom for Quality Control of MRI Knee Measurements in Clinical Trials.
    D. L. White, M. Kotari, R. A. D. Carano
    2000

Ellen Filvaroff, Senior Scientist and Alex Avardo, Receipent of Free Medicine From the Genentech® Access to Care Foundation, on the cover of Genentech's 2006 Annual Report.