Harinder Singh

Staff Scientist, Immunology

POSTDOC MENTOR

"There is a distinct sense of fulfillment that derives from creating a device or a drug with a real-world impact."

• 23
  years at Genentech
• 11
  publications (2010-12)
• 2
  awards & honors

Deep down the reason that I was attracted to join Genentech is because it attempts to combine the best elements of an academic culture with those from the translational research and commercial domains. It represents an innovative and focused organization with a vital biomedical mission. As a second-generation molecular biologist, I maintain a strong interest in the rich history of biotechnology. Therefore, I was also drawn to Genentech because of its central position in spawning the generation of biopharmaceuticals after the advent of recombinant DNA. Being at Genentech not only enables me to continue my creative research endeavors, but also challenges me to seek a deeper understanding of immunological disease processes and direct the development of novel and highly effective therapeutics.

• My Focus

  My laboratory is assembling and analyzing gene regulatory networks that orchestrate the development of various cell types of the innate and adaptive immune system including B-and T lymphocytes, macrophages, neutrophils dendritic and mast cells. These networks are comprised of inter-connected transcription factors, signaling molecules and miRNAs that regulate cell fate choice and developmental transitions. We are currently utilizing a combination of genetic, molecular and mathematical modeling approaches to further analyze such gene regulatory networks. These include: ChIPseq to connect each transcription factor in a given network with its large set of target genes and high throughput functional screens of lineage- and stage-specific cis-regulatory elements.

  We are also interested in manipulating these regulatory networks to engineer stem cells to efficiently adopt particular immune cell fates. Over the past few years I have been shifting the focus of some of our basic research from analyzing molecular circuits that control the development of innate and adaptive immune cells to those that control their activation and function in the context of an immune response.

  The move to Genentech is greatly accelerating this process. In the final analysis we seek not only to advance knowledge and understanding in this field but to effectively harness it to enhance the human condition by ameliorating or inhibiting immune-mediated disease processes.

• Publications

  • Compensatory dendritic cell development mediated by BATF-IRF interactions

    Nature, 2012, ISSN: 0028-0836

    Tussiwand, Roxane; Lee, Wan-Ling; Murphy, Theresa L.; Mashayekhi, Mona; Kc, Wumesh; Albring, Jörn C.; Satpathy, Ansuman T.; Rotondo, Jeffrey A.; Edelson, Brian T.; Kretzer, Nicole M.; Wu, Xiaodi; Weiss, Leslie A.; Glasmacher, Elke; Li, Peng; Liao, Wei; Behnke, Michael; Lam, Samuel S. K.; Aurthur, Cora T.; Leonard, Warren J.; Singh, Harinder; Stallings, Christina L.; David Sibley, L.; Schreiber, Robert D.; Murphy, Kenneth M.

     View on PubMed

  • A Genomic Regulatory Element That Directs Assembly and Function of Immune-Specific AP-1-IRF Complexes

    Science, 2012, ISSN: 0036-8075

    Glasmacher, Elke; Agrawal, Smita; Chang, Abraham B; Murphy, Theresa L; Zeng, Wenwen; Vander Lugt, Bryan; Khan, Aly A; Ciofani, Maria; Spooner, Chauncey; Rutz, Sascha; Hackney, Jason; Nurieva, Roza; Escalante, Carlos R; Ouyang, Wenjun; Littman, Dan R; Murphy, Kenneth M; Singh, Harinder

     View on PubMed

  • Elevated and sustained expression of the transcription factors Egr1 and Egr2 controls NKT lineage differentiation in response to TCR signaling

    Nature Immunology, 2012, ISSN: 1529-2908

    Seiler, Michael P.; Mathew, Rebecca; Liszewski, Megan K.; Spooner, Chauncey; Barr, Kenneth; Meng, Fanyong; Singh, Harinder; Bendelac, Albert

     View on PubMed

  • A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation

    Nature Immunology, 2012, ISSN: 1529-2908

    Ochiai, Kyoko; Maienschein-Cline, Mark; Mandal, Malay; Triggs, Joseph R.; Bertolino, Eric; Sciammas, Roger; Dinner, Aaron R.; Clark, Marcus R.; Singh, Harinder

     View on PubMed

  • The A-MYSM Power of Deubiquitinases

    Immunity, 23 Dec 2011, v35(6), p847-849, ISSN: 1074-7613

    Demarco, Ignacio A.; Singh, Harinder

     View on PubMed

  • Transcription factor c-Maf mediates the TGF-beta-dependent suppression of IL-22 production in T(H)17 cells.

    Nature immunology, 16 Oct 2011, ISSN: 1529-2916.

    Rutz, Sascha; Noubade, Rajkumar; Eidenschenk, Celine; Ota, Naruhisa; Zeng, Wenwen; Zheng, Yan; Hackney, Jason; Ding, Jiabing; Singh, Harinder; Ouyang, Wenjun

     View on PubMed

  • Transcription factor c-Maf mediates the TGF-β-dependent suppression of IL-22 production in T(H)17 cells

    Nature Immunology, 2011, ISSN: 0022-1767

    Rutz, Sascha; Noubade, Rajkumar; Eidenschenk, Celine; Singh, Harinder; Ouyang, Wenjun

  • IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.

    Nature Immunology, 12 Oct 2011, ISSN: 1529-2916.

    Ramirez, Carrozzi Vladimir; Sambandam, Arivazhagan; Luis, Elizabeth; Lin, Zhongua; Jeet, Surinder; Lesch, Justin; Hackney, Jason; Kim, Janice; Zhou, Meijuan; Lai, Joyce; Modrusan, Zora; Sai, Tao; Lee, Wyne; Xu, Min; Caplazi, Patrick; Diehl, Lauri; de Voss, Jason; Balazs, Mercedesz; Gonzalez, Lino Jr; Singh, Harinder; Ouyang, Wenjun; Pappu, Rajita

     View on PubMed

  • Cooperation between deficiencies of IRF-4 and IRF-8 promotes both myeloid and lymphoid tumorigenesis.

    Blood, 2010, ISSN: 0006-4971

    Jo, S.H.; Schatz, J.H.; Acquaviva, J.; Singh, H.; Ren, R.

  • IL-7 signaling and the control of B cell development.

    Cytokine, 2010, ISSN: 1043-4666

    Singh, H.

  • Double TIP-ping.

    Science, 2010, ISSN: 1095-9203

    Singh, H.; Demarco, I.

• Education
  • Center for Cancer Research, Massachusetts Institute of Technology, Postdoctoral Fellow – 1988
  • Northwestern University Molecular Biology and Cell Biology, Ph.D. – 1984
  • Punjab Agricultural University, M.Sc./B.Sc. – 1979
• Awards & Honors
  • Postdoctoral fellowship, The Jane Coffin Childs Memorial Fund for Medical Research – 1987
  • Thapar Gold Medal, Punjab Agricultural University, Outstanding University Undergraduate – 1977