Lino Gonzalez

Scientist, Protein Chemistry

POSTDOC MENTOR

"The thrill of discovery is a great motivation and, being at a place like Genentech, these discoveries can directly impact the development of new therapeutics."

• 12
  years at Genentech
• 10
  publications (2010-12)
• 6
  awards & honors

I joined Genentech in 2001 for the simple reason that it is one of the best places in the world to do science. The scientific resources, infrastructure and people are unparalleled. This creates a fertile ground for quickly moving a project from an idea to a reality. It was also clear to me that this was a great place to do science by the number of scientists that had spent a majority of their careers working here.

• My Focus

  My lab focuses on identifying novel receptor-ligand or co-receptor interactions. Now that the human genome is complete, one of the next steps is to understand the interactions between all transmembrane and secreted proteins, which represent about one-third of all human genes. This knowledge would greatly aid in characterizing the biological function of many receptors/ligand pairs and their potential as therapeutic targets. We concentrate on receptors of the immune system, in particular the immunoglobulin receptor family.

  Surprisingly, a significant number of these receptors still remain orphans. In order to identify candidate partners, we have utilized Genentech's SPDI (Secreted Protein Discovery Initiative) protein library, consisting of over 1,000 purified, secreted proteins. Currently, we are using surface plasmon resonance (SPR) and Bio-layer interferometry (BLI) technology to screen hundreds of interactions per day. To date, this work has led to the identification of several novel interactions that are now being evaluated for their therapeutic potential.

• Publications

  • A requirement for wild-type Ras isoforms in mutant KRas-driven signalling and transformation

    Biochemical Journal, 2013, ISSN: 0264-6021

    Bentley, Carolyn; Jurinka, Stefanie S; Kljavin, Noelyn M; Vartanian, Steffan; Ramani, Sree R; Gonzalez, Lino C; Yu, Kebing; Modrusan, Zora; Du, Pan; Bourgon, Richard; Neve, Richard M; Stokoe, David

     View on PubMed

  • Protein microarrays for identification of novel extracellular protein-protein interactions

    Current Protocols in Protein Science, 2013, ISSN: 1934-3655

    Tom, Irene; Lewin-Koh, Nicholas; Ramani, Sree R; Gonzalez, Lino C

     View on PubMed

  • Critical role of activation induced cytidine deaminase in Experimental Autoimmune Encephalomyelitis

    Autoimmunity, 2012, ISSN: 0891-6934

    Sun, Yonglian; Peng, Ivan; Senger, Kate; Hamidzadeh, Kajal; Reichelt, Mike; Baca, Miriam; Yeh, Ronald; Lorenzo, Maria N; Sebrell, Andrew; Cruz, Christopher Dela; Tam, Lucinda; Corpuz, Racquel; Wu, Jiansheng; Sai, Tao; Roose-Girma, Merone; Warming, Søren; Balazs, Mercedesz; Gonzalez, Lino C; Caplazi, Patrick; Martin, Flavius; Devoss, Jason; Zarrin, Ali A

     View on PubMed

  • Protein microarrays, biosensors, and cell-based methods for secretome-wide extracellular protein-protein interaction mapping

    Methods, 2012, ISSN: 1046-2023

    Gonzalez, Lino;

     View on PubMed

  • Evolutionarily conserved paired immunoglobulin-like receptor α (PILRα) domain mediates its interaction with diverse sialylated ligands

    Journal of Biological Chemistry, 2012, ISSN: 0021-9258

    Sun, Yonglian; Senger, Kate; Baginski, Tomasz K; Mazloom, Anita; Chinn, Yvonne; Pantua, Homer; Hamidzadeh, Kajal; Ramani, Sree Ranjani; Luis, Elizabeth; Tom, Irene; Sebrell, Andrew; Quinones, Gabriel; Ma, Yan; Mukhyala, Kiran; Sai, Tao; Ding, Jiabing; Haley, Benjamin; Shadnia, Hooman; Kapadia, Sharookh B; Gonzalez, Lino C; Hass, Philip E; Zarrin, Ali A

     View on PubMed

  • An evolutionary conserved PILRα domain mediates its interaction with diverse sialylated ligands

    Journal of Biological Chemistry, 2012, ISSN: 0021-9258

    Sun, Yonglian; Senger, Kate; Baginski, Tomasz K; Mazloom, Anita; Chinn, Yvonne; Pantua, Homer; Hamidzadeh , Kajal; Ramani, Sree Ranjani; Luis, Elizabeth; Tom, Irene; Sebrell, Andrew; Quinones, Gabriel; Ma, Yan; Mukhyala, Kiran; Sai, Tao; Ding, Jiabing; Haley, Benjamin; Shadnia, Hooman; Kapadia, Sharookh B; Gonzalez, Lino C; Hass, Philip E; Zarrin, Ali A;

     View on PubMed

  • IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.

    Nature Immunology, 12 Oct 2011, ISSN: 1529-2916.

    Ramirez, Carrozzi Vladimir; Sambandam, Arivazhagan; Luis, Elizabeth; Lin, Zhongua; Jeet, Surinder; Lesch, Justin; Hackney, Jason; Kim, Janice; Zhou, Meijuan; Lai, Joyce; Modrusan, Zora; Sai, Tao; Lee, Wyne; Xu, Min; Caplazi, Patrick; Diehl, Lauri; de Voss, Jason; Balazs, Mercedesz; Gonzalez, Lino Jr; Singh, Harinder; Ouyang, Wenjun; Pappu, Rajita

     View on PubMed

  • A secreted protein microarray platform for extracellular protein interaction discovery.

    Analytical Biochemistry, 21 Sep 2011, ISSN: 1096-0309.

    Ramani, Sree R; Tom, Irene; Lewin, Koh Nicholas; Wranik, Bernd; Depalatis, Laura; Zhang, Jianjun; Eaton, Dan; Gonzalez, Lino C

     View on PubMed

  • Antibodies specific for a segment of human membrane IgE deplete IgE-producing B cells in humanized mice.

    Journal of Clinical Investigation, 2010, ISSN: 1558-8238

    Brightbill, H.; Jeet, S.; Lin, Z.; Yan, D.; Zhou, M.; Tan, M.; Nguyen, A.; Yeh, S.; Delarosa, D.; Leong, S.; Wong, T.; Chen, Y.; Ultsch, M.; Luis, E.; Ramani, S.; Jackman, J.; Gonzalez, L.; Dennis, M.; Chuntharapai, A.; DeForge, L.; Meng, Y.; Xu, M.; Eigenbrot, C.; Lee, W.; Refino, C.; Balazs, M.; Wu, L.

  • The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

    Nature Immunology, 2009, ISSN: 1529-2916

    Yu, X.; Harden, K.; Gonzalez, L.C.; Francesco, M.; Chiang, E.; Irving, B.; Tom, I.; Ivelja, S.; Refino, C.J.; Clark, H.; Eaton, D.; Grogan, J.L.

• Education
  • Stanford University, California, Postdoctoral Fellow – 2001
  • Yale University, Postdoctoral Fellow – 1997
  • University of California, Berkeley, Department of Molecular and Cell Biology, Ph.D. – 1996
  • University of California, Santa Barbara, Department of Chemistry, B.S. – 1991
• Awards & Honors
  • Howard Hughes Medical Institute Postdoctoral Research Fellow – 2001
  • National Science Foundation Minority Postdoctoral Research Fellow – 1999
  • Howard Hughes Medical Institute Postdoctoral Research Fellow – 1997
  • Patricia Robert Harris Fellowship for Graduate Studies – 1994
  • Summer Research Fellowship, University of California, Santa Cruz – 1991
  • Undergraduate Research Grant Award, University of California, Santa Barbara – 1990