South San Francisco
California, United States of America
A postdoctoral position is available in the Department of Early Discovery Biochemistry to investigate protein – protein interactions and develop peptide probes for modulating targets of therapeutic interest by utilizing biochemistry and structural biology approaches. The group utilizes a multidisciplinary molecular approach for peptide discovery and target validation, encompassing diversity methods such as phage display. Projects will focus on molecular understanding of new targets in cancer and stem cell biology and dissecting their biochemical mechanisms of regulation. Other projects include the development of cell permeable peptides and characterization of their cellular trafficking routes.
Successful candidates will be highly motivated to work independently in a collaborative environment. Candidates must have a Ph.D. in biochemistry, cell biology or related discipline. Candidates should have experience in protein chemistry, cellular biochemistry and state of the art molecular biology techniques as demonstrated by a first-author paper published or accepted in a peer-reviewed journal. A strong quantitative background and demonstrated expertise in recombinant protein expression and purification, protein engineering, structural biology, analytical and biophysical methods, bioconjugation reactions, and phage diversity methods is desirable. Experience with tissue culture, cell-based assays and transfections, gene knockdown, gene expression analysis and fluorescence microscopy is a plus. The candidate must have strong analytical, collaborative, communication and organizational skills.
A Nile, F de Sousa E Melo, S Mukund, R Piskol, S Hansen, L Zhou, Y Zhang, Y Fu, E Gogol, L Kömüves, ZModrusan, S Angers, Y Franke, C Koth, W Fairbrother, W Wang, F de Sauvage, RN Hannoush. A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells. Nature Chemical Biology 2018, 14, 582-590.
A Nile, S Mukund, K Stanger W Wang and RN Hannoush. Unsaturated fatty acyl recognition by Frizzled receptors mediates dimerization upon Wnt binding. PNAS 2017, 114, 4147-4152.
X Gao, K Stanger, H Kaluarachchi H, T Maurer, P Ciepla, C Chalouni, Y Franke, RN Hannoush. Cellular uptake of a cystine-knot peptide and modulation of its intracellular trafficking. Sci Rep. 2016, 6:35179.
K Stanger, T Maurer , H Kaluarachchi, M Coons, Y Franke, RN Hannoush. Backbone cyclization of a recombinant cystine-knot peptide by engineered Sortase A. FEBS Lett. 2014, 588, 4487-96.
X. Gao, RN Hannoush, Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine. Nature Chemical Biology 2014, 10, 61-68.
RN Hannoush, JL Sun, The chemical toolbox for monitoring protein fatty acylation and prenylation. Nature Chemical Biology 2010, 6, 498 - 506.
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