Brad Snedecor
Distinguished Engineer, Early Stage Cell Culture
Staff Scientist
40
Years at Genentech
37
Publications
2
Awards & Honors
I came to Genentech in 1981 as a newly minted Biochemical Engineer and was hired as a Scientist to help establish the Biocatalysis Department then in Research, and in 1987 joined Medicinal and Biomolecular Chemistry. In 1988 I moved into Fermentation R&D, developing the initial fermentation process technology for antibody fragment expression in E. coli, and from 1994 led the E. coli fermentation development labs for the next decade. Since that time I have focused on mammalian cell culture technology development, and now lead an Early Stage Cell Culture group in Pharma Technical Development that develops mammalian manufacturing cell lines and works to improve cell line development.
Pharma Technical Development bridges Research to Manufacturing, and I enjoy tremendously the opportunity to engage the diverse problems that entails, working with diverse and ingenious people across the company.
Featured Publication
It0s time to regulate: Coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.
Biotechnol Prog. 2014 Aug 8. [Epub ahead of print]
Misaghi S, Chang J, Snedecor B.
My Focus
The main focus of my labs is to create stable mammalian cell lines for the manufacturing of our pipeline products. Technical development research in the labs focuses on improvement of the methods we use to create cell lines, and on understanding and improving the lines themselves. Current topics include regulated expression of toxic proteins, improvement of targeted integration sufficient to support manufacturing cell line creation, and improved methods for high throughput single cell cloning.
Publications
Journal of Biotechnology, ISSN: 18734863 01681656
Salina Louie; Jayanthi Lakkyreddy; Brian M. Castellano; Benjamin Haley; Anh Nguyen Dang; Cynthia Lam; Danming Tang; Steven E. Lang; Brad Snedecor; Shahram Misaghi
Journal of Cell Biology, ISSN: 15408140
Danming Tang; Wendy Sandoval; Cynthia Lam; Benjamin Haley; Peter Liu; Di Xue; Deepankar Roy; Tom Patapoff; Salina Louie; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033 87567938
Joe Carver; Domingos Ng; Michelle Zhou; Peggy Ko; Dejin Zhan; Mandy Yim; David Shaw; Brad Snedecor; Mike Laird; Steven E. Lang; Amy Shen; Zhilan Hu
Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules
Biotechnology Journal, ISSN: 18606768
Emily Dong; Cynthia Lam; Danming Tang; Salina Louie; Mandy Yim; Ambrose Williams; William S. Sawyer; Shirley Yip; Joe Carver; Ali AlBarakat; Joni Tsukuda; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033 87567938
Salina Louie; Amy Heidersbach; Noelia Blanco; Benjamin Haley; Christopher M. Rose; Peter Liu; Mandy Yim; Danming Tang; Cynthia Lam; Wendy Sandoval; David Shaw; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033 87567938
Tomofumi Tadauchi; Cynthia Lam; Laura Liu; Yizhou Zhou; Danming Tang; Salina Louie; Brad Snedecor; Shahram Misaghi
Biotechnology Journal, ISSN: 18607314 18606768
Meg Tung; Danming Tang; Szu-Han Wang; Dejin Zhan; Karen Kiplinger; Shu Pan; Yifeng Jing; Amy Shen; Patrick Ahyow; Brad Snedecor; Martin Gawlitzek; Shahram Misaghi
Biotechnology Journal, ISSN: 18607314 18606768
Danming Tang; Jayashree Subramanian; Benjamin Haley; Jordan Baker; Lucas Luo; Wendy Hsu; Peter Liu; Wendy Sandoval; Mike Laird; Brad Snedecor; Masaru Shiratori; Shahram Misaghi
Biotechnology and Bioengineering, ISSN: 10970290
Salina Louie; Benjamin Haley; Brett Marshall; Amy Heidersbach; Mandy Yim; Martina Brozynski; Danming Tang; Cynthia Lam; Bronia Petryniak; David (ESCC) Shaw; Jeongsup Shim; Aaron Miller; John B. Lowe (Path); Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033 87567938
David (ESCC) Shaw; Mandy Yim; Joni Tsukuda; John Joly; Andy Lin; Brad Snedecor; Mike Laird; Steven E. Lang
Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells
Biotechnology Progress, ISSN: 87567938
Zhilan Hu; Danming Tang; Shahram Misaghi; Guoying Jiang; Christopher Yu (PAC); Mandy Yim; David (ESCC) Shaw; Brad Snedecor; Mike Laird; Amy Shen
Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality
Biotechnology Progress, ISSN: 87567938
Peggy Ko; Shahram Misaghi; Zhilan Hu; Dejin Zhan; Joni Tsukuda; Mandy Yim; Mark Sanford; David (ESCC) Shaw; Masaru Shiratori; Brad Snedecor; Mike Laird; Amy Shen
Biotechnology Journal, ISSN: 18607314 18606768
Danming Tang; Cynthia Lam; Salina Louie; Kam Hon Hoi; David (ESCC) Shaw; Mandy Yim; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033
Cynthia Lam; Lydia Santell; Blair Wilson; Mandy Yim; Salina Louie; Danming Tang; David (ESCC) Shaw; Pamela Chan; Bob Lazarus; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033
Zhilan Hu; Wendy Hsu; Abby Pynn; Domingos Ng; Donna Quicho; Yilma Adem; Zephie Kwong; Jacob (Brad) Mauger; John Joly; Brad Snedecor; Mike Laird; Dana Andersen; Amy Shen
Biotechnology Progress, ISSN: 15206033 87567938
Yizhou Zhou; David (ESCC) Shaw; Cynthia Lam; Joni Tsukuda; Mandy Yim; Danming Tang; Salina Louie; Mike Laird; Brad Snedecor; Shahram Misaghi
Biotechnology and Bioengineering, ISSN: 10970290
Zhilan Hu; Henry Zhang; Benjamin Haley; Frank Macchi; Feng Yang; Shahram Misaghi; Joseph Elich; Renee Yang; Yun Tang; John Joly; Brad Snedecor; Amy Shen
Biotechnology Progress, ISSN: 15206033
Shahram Misaghi; David (ESCC) Shaw; Salina Louie; Adrian Nava; Laura Simmons; Brad Snedecor; CK Poon; Jonathan Paw; Laurie Appling; Jim Cupp
Molecular Biotechnology, ISSN: 15590305
Shirley Yip; Michelle Zhou; John Joly; Brad Snedecor; Amy Shen; Yongping Crawford
Biotechnology Progress, ISSN: 15206033
Shahram Misaghi; Jennifer Chang; Brad Snedecor
Biotechnology Progress, ISSN: 87567938
Yongping Crawford; Michelle Zhou; Zhilan Hu; John Joly; Brad Snedecor; Amy Shen; Albert Gao
Biotechnology Progress, ISSN: 87567938
Zhilan Hu; Donglin Guo; Shirley Yip; Dejin Zhan; Shahram Misaghi; John Joly; Brad Snedecor; Amy Shen
Biotechnology Progress, ISSN: 87567938
Shahram Misaghi; Yan Qu; Andrew Snowden; Jennifer Chang; Brad Snedecor
Biotechnology and Bioengineering, ISSN: 00063592
Inn Yuk; Boyan Zhang; Yi Yang; George Dutina; Kim Leach; Natu Vijayasankaran; Amy Shen; Dana Andersen; Brad Snedecor; John Joly
Journal of Biotechnology, ISSN: 01681656
Michelle Zhou; Yongping Crawford; Domingos Ng; Jack Tung; Abby Pynn; Angela Meier; Inn Yuk; Natu Vijayasankaran; Kim Leach; John Joly; Brad Snedecor; Amy Shen
Biotechnology and Bioengineering, ISSN: 00063592
Donglin Guo; Albert Gao; David Michels; Lauren Brown; Marian Eng; Betty Chan; Mike Laird; Boyan Zhang; Christopher Yu (PAC); John Joly; Brad Snedecor; Amy Shen
Metabolic Engineering, ISSN: 10967176
Adam Meadows; Rahi Karnik; Harry Lam; Sean Forestell; Brad Snedecor
Biotechnology and Bioengineering, ISSN: 00063592
Christina Chen; Brad Snedecor; Julie Nishihara; John Joly; Nancy McFarland; Dana Andersen; John Battersby; Katy Champion
Cytokine, ISSN: 10434666
Steve Leong; Laura DeForge; Leonard G. Presta; Tania Gonzalez; Audrey L. Fan; Marcel Reichert; Anan Chuntharapai; Jin Kim; Daniel B. Tumas; Wyne Lee; Peter Gribling; Brad Snedecor; Han F. Chen; Vanessa Hsei; Monika B Schoenhoff; Victoria Grace Hale; James F. Deveney; Iphigenia-Leonidou Koumenis; Zahra Shahrokh; McKay; Walter Galan; Brian Wagner; Daljit Narindray; Hébert; Gerardo A. Zapata
Biotechnology and Bioengineering, ISSN: 00063592
Dana Andersen; James R. Swartz; Thomas Ryll; Lin; Brad Snedecor
Journal of Chromatography A, ISSN: 00219673
John Battersby; Brad Snedecor; Christina Chen; Katy Champion; Lavon L. Riddle; Martin Vanderlaan
Bio/Technology, ISSN: 0733222X
Zhu; Gerardo A. Zapata; M. Refaat REFAAT Shalaby; Brad Snedecor; Man Chert; Paul Carter
Journal of Immunology, ISSN: 00221767
Maria Inês Rodrigues; Brad Snedecor; Christina Chen; Wai Lee Wong; Shaily Garg; Greg Blank; Maneval; Paul Carter
Biochemistry, ISSN: 00062960
Robert Kelley; Mark O'Connell; Paul Carter; Leonard G. Presta; Charles Eigenbrot; Michael S. Covarrubias; Brad Snedecor; James H. Bourell; David Vetterlein
Bio/Technology, ISSN: 0733222X
Paul Carter; Robert Kelley; Maria Inês Rodrigues; Brad Snedecor; Michael S. Covarrubias; Mark Velligan; Wai T. Wong Lee; Ann Rowland; Claire E. Kotts; Monique E. Carver; Maria Yang; James H. Bourell; Michael Shepard; Dennis J. Henner
Journal of Bacteriology, ISSN: 00219193
Brad Snedecor; Chu; Ellson Chen
Org Chem from Biomass,
Brad Snedecor; Gomez
Education
- Massachusetts Institute of Technology, Ph.D in Biochemical Engineering with a minor in Molecular Biology – 1981
- Massachusetts Institute of Technology, M.S in Biochemcal Engineering – 1977
- Massachusetts Institute of Technology, B.S. in Chemistry – 1975
Awards & Honors
- National Science Foundation Graduate Fellow – 1976-1979
- National Merit Scholar – 1971-1975