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Scientists / Our Scientists

Brad Snedecor

Distinguished Engineer, Early Stage Cell Culture

37

Years at Genentech

29

Publications

2

Awards & Honors

I came to Genentech in 1981 as a newly minted Biochemical Engineer and was hired as a Scientist to help establish the Biocatalysis Department then in Research, and in 1987 joined Medicinal and Biomolecular Chemistry. In 1988 I moved into Fermentation R&D, developing the initial fermentation process technology for antibody fragment expression in E. coli, and from 1994 led the E. coli fermentation development labs for the next decade. Since that time I have focused on mammalian cell culture technology development, and now lead an Early Stage Cell Culture group in Pharma Technical Development that develops mammalian manufacturing cell lines and works to improve cell line development.

Pharma Technical Development bridges Research to Manufacturing, and I enjoy tremendously the opportunity to engage the diverse problems that entails, working with diverse and ingenious people across the company.

Featured Publication

It's time to regulate: Coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.

Biotechnol Prog. 2014 Aug 8. [Epub ahead of print]

Misaghi S, Chang J, Snedecor B.

View Abstract on PubMed

The main focus of my labs is to create stable mammalian cell lines for the manufacturing of our pipeline products. Technical development research in the labs focuses on improvement of the methods we use to create cell lines, and on understanding and improving the lines themselves. Current topics include regulated expression of toxic proteins, improvement of targeted integration sufficient to support manufacturing cell line creation, and improved methods for high throughput single cell cloning.

Taming hyperactive hDNase I: Stable inducible expression of a hyperactive salt- and actin-resistant variant of human deoxyribonuclease I in CHO cells

Biotechnology Progress, ISSN: 15206033

Cynthia Lam; Lydia Santell; Blair Wilson; Mandy Yim; Salina Louie; Danming Tang; David (ESCC) Shaw; Pamela Chan; Bob Lazarus; Brad Snedecor; Shahram Misaghi

Carboxypeptidase D is the only enzyme responsible for antibody C-terminal lysine cleavage in Chinese hamster ovary (CHO) cells

Biotechnology and Bioengineering, ISSN: 10970290

Zhilan Hu; Henry Zhang; Benjamin Haley; Frank Macchi; Feng Yang; Shahram Misaghi; Joseph Elich; Renee Yang; Yun Tang; John Joly; Brad Snedecor; Amy Shen

FX knockout CHO hosts can express desired ratios of fucosylated or afucosylated antibodies with high titers and comparable product quality

Biotechnology and Bioengineering, ISSN: 10970290

Salina Louie; Benjamin Haley; Brett Marshall; Amy Heidersbach; Mandy Yim; Martina Brozynski; Danming Tang; Cynthia Lam; Bronia Petryniak; David (ESCC) Shaw; Jeongsup Shim; Aaron Miller; John B. Lowe (Path); Brad Snedecor; Shahram Misaghi

Slashing the timelines: Opting to generate high-titer clonal lines faster via viability-based single cell sorting

Biotechnology Progress, ISSN: 15206033

Shahram Misaghi; David (ESCC) Shaw; Salina Louie; Adrian Nava; Laura Simmons; Brad Snedecor; CK Poon; Jonathan Paw; Laurie Appling; Jim Cupp

Complete knockout of the lactate dehydrogenase A gene is lethal in pyruvate dehydrogenase kinase 1, 2, 3 down-regulated CHO cells

Molecular Biotechnology, ISSN: 15590305

Shirley Yip; Michelle Zhou; John Joly; Brad Snedecor; Amy Shen; Yongping Crawford

It's time to regulate: Coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression

Biotechnology Progress, ISSN: 15206033

Shahram Misaghi; Jennifer Chang; Brad Snedecor

Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined φC31 integrase and CRE-Lox technologies

Biotechnology Progress, ISSN: 87567938

Yongping Crawford; Michelle Zhou; Zhilan Hu; John Joly; Brad Snedecor; Amy Shen; Albert Gao

Chinese hamster ovary K1 host cell enables stable cell line development for antibody molecules which are difficult to express in DUXB11-derived dihydrofolate reductase deficient host cell

Biotechnology Progress, ISSN: 87567938

Zhilan Hu; Donglin Guo; Shirley Yip; Dejin Zhan; Shahram Misaghi; John Joly; Brad Snedecor; Amy Shen

Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production

Biotechnology Progress, ISSN: 87567938

Shahram Misaghi; Yan Qu; Andrew Snowden; Jennifer Chang; Brad Snedecor

Controlling glycation of recombinant antibody in fed-batch cell cultures

Biotechnology and Bioengineering, ISSN: 00063592

Inn Yuk; Boyan Zhang; Yi Yang; George Dutina; Kim Leach; Natu Vijayasankaran; Amy Shen; Dana Andersen; Brad Snedecor; John Joly

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

Journal of Biotechnology, ISSN: 01681656

Michelle Zhou; Yongping Crawford; Domingos Ng; Jack Tung; Abby Pynn; Angela Meier; Inn Yuk; Natu Vijayasankaran; Kim Leach; John Joly; Brad Snedecor; Amy Shen

Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells

Biotechnology and Bioengineering, ISSN: 00063592

Donglin Guo; Albert Gao; David Michels; Lauren Brown; Marian Eng; Betty Chan; Mike Laird; Boyan Zhang; Christopher Yu (PAC); John Joly; Brad Snedecor; Amy Shen

Application of dynamic flux balance analysis to an industrial Escherichia coli fermentation

Metabolic Engineering, ISSN: 10967176

Adam Meadows; Rahi Karnik; Harry Lam; Sean Forestell; Brad Snedecor

High-Level Accumulation of a Recombinant Antibody Fragment in the Periplasm of Escherichia coli Requires a Triple-Mutant (degP prc spr) Host Strain

Biotechnology and Bioengineering, ISSN: 00063592

Christina Chen; Brad Snedecor; Julie Nishihara; John Joly; Nancy McFarland; Dana Andersen; John Battersby; Katy Champion

Adapting pharmacokinetic properties of a humanized anti-interleukin-8 antibody for therapeutic applications using site-specific pegylation

Cytokine, ISSN: 10434666

Steve Leong; Laura DeForge; Leonard G. Presta; Tania Gonzalez; Audrey L. Fan; Marcel Reichert; Anan Chuntharapai; Jin Kim; Daniel B. Tumas; Wyne Lee; Peter Gribling; Brad Snedecor; Han F. Chen; Vanessa Hsei; Monika B Schoenhoff; Victoria Grace Hale; James F. Deveney; Iphigenia-Leonidou Koumenis; Zahra Shahrokh; McKay; Walter Galan; Brian Wagner; Daljit Narindray; Hébert; Gerardo A. Zapata

Metabolic oscillations in an E. coli fermentation

Biotechnology and Bioengineering, ISSN: 00063592

Dana Andersen; James R. Swartz; Thomas Ryll; Lin; Brad Snedecor

Affinity-reversed-phase liquid chromatography assay to quantitate recombinant antibodies and antibody fragments in fermentation broth

Journal of Chromatography A, ISSN: 00219673

John Battersby; Brad Snedecor; Christina Chen; Katy Champion; Lavon L. Riddle; Martin Vanderlaan

High level secretion of a humanized bispecific diabody from Escherichia coli

Bio/Technology, ISSN: 0733222X

Zhu; Gerardo A. Zapata; M. Refaat REFAAT Shalaby; Brad Snedecor; Man Chert; Paul Carter

Engineering Fab' fragments for efficient F(ab)2 formation in Escherichia coli and for improved in vivo stability

Journal of Immunology, ISSN: 00221767

Maria Inês Rodrigues; Brad Snedecor; Christina Chen; Wai Lee Wong; Shaily Garg; Greg Blank; Maneval; Paul Carter

Antigen binding thermodynamics and antiproliferative effects of chimeric and humanized anti-p185(HER2) antibody Fab fragments

Biochemistry, ISSN: 00062960

Robert Kelley; Mark O'Connell; Paul Carter; Leonard G. Presta; Charles Eigenbrot; Michael S. Covarrubias; Brad Snedecor; James H. Bourell; David Vetterlein

High level Escherichia coli expression and production of a bivalent humanized antibody fragment

Bio/Technology, ISSN: 0733222X

Paul Carter; Robert Kelley; Maria Inês Rodrigues; Brad Snedecor; Michael S. Covarrubias; Mark Velligan; Wai T. Wong Lee; Ann Rowland; Claire E. Kotts; Monique E. Carver; Maria Yang; James H. Bourell; Michael Shepard; Dennis J. Henner

Selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of Peptostreptococcus asaccharolyticus

Journal of Bacteriology, ISSN: 00219193

Brad Snedecor; Chu; Ellson Chen

PROSPECTS FOR THE USE OF GENETIC ENGINEERING TO PRODUCE INDUSTRIAL CHEMICALS WITH STRICT ANAEROBES.

Org Chem from Biomass,

Brad Snedecor; Gomez

Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells

Biotechnology Progress, ISSN: 87567938

Zhilan Hu; Danming Tang; Shahram Misaghi; Guoying Jiang; Christopher Yu (PAC); Mandy Yim; David (ESCC) Shaw; Brad Snedecor; Mike Laird; Amy Shen

A strategy to accelerate protein production from a pool of clones in Chinese hamster ovary cells for toxicology studies

Biotechnology Progress, ISSN: 15206033

Zhilan Hu; Wendy Hsu; Abby Pynn; Domingos Ng; Donna Quicho; Yilma Adem; Zephie Kwong; Jacob (Brad) Mauger; John Joly; Brad Snedecor; Mike Laird; Dana Andersen; Amy Shen

Supplementation of Nucleosides During Selection can Reduce Sequence Variant Levels in CHO Cells Using GS/MSX Selection System

Biotechnology Journal, ISSN: 18607314 18606768

Danming Tang; Cynthia Lam; Salina Louie; Kam Hon Hoi; David (ESCC) Shaw; Mandy Yim; Brad Snedecor; Shahram Misaghi

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not

Biotechnology Progress, ISSN: 15206033 87567938

Yizhou Zhou; David (ESCC) Shaw; Cynthia Lam; Joni Tsukuda; Mandy Yim; Danming Tang; Salina Louie; Mike Laird; Brad Snedecor; Shahram Misaghi

Development and characterization of an automated imaging workflow to generate clonally-derived cell lines for therapeutic proteins

Biotechnology Progress, ISSN: 15206033 87567938

David (ESCC) Shaw; Mandy Yim; Joni Tsukuda; John Joly; Andy Lin; Brad Snedecor; Mike Laird; Steven E. Lang

Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality

Biotechnology Progress, ISSN: 87567938

Peggy Ko; Shahram Misaghi; Zhilan Hu; Dejin Zhan; Joni Tsukuda; Mandy Yim; Mark Sanford; David (ESCC) Shaw; Masaru Shiratori; Brad Snedecor; Mike Laird; Amy Shen

Massachusetts Institute of Technology, Ph.D in Biochemical Engineering with a minor in Molecular Biology – 1981
Massachusetts Institute of Technology, M.S in Biochemcal Engineering – 1977
Massachusetts Institute of Technology, B.S. in Chemistry – 1975

National Science Foundation Graduate Fellow – 1976-1979
National Merit Scholar – 1971-1975