David Castillo-Azofeifa - Principal Scientist, Regenerative Medicine

David Castillo-Azofeifa

Principal Scientist, Regenerative Medicine

"At Genentech, helping patients becomes a reality through innovation and high performing teamwork."
Years at Genentech
Awards & Honors

I am a stem cell and developmental biologist interested in the fundamental processes underlying tissue regeneration and degeneration. My lab aims to elucidate the signaling circuits that regulate the interaction between stem cells and their surroundings, which are responsible for homeostasis and regeneration. We test our hypothesis using our expertise in developing and applying genetic and pharmacology tools to manipulate cell decisions with unprecedented regional, cell type, and temporal specificity. Our ultimate goal is to utilize fundamental discoveries to develop therapeutics to regenerate and rejuvenate epithelial tissues, including the intestine, during disease-driven injury and aging.

Featured Publication

Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis.

J Clin Invest. 2023.

Reyes EA§, Castillo-Azofeifa D§, Rispal J§, Wald T, Zwick RK, Palikuqi B, Mujukian A, Rabizadeh S, Gupta AR, Gardner JM, Boffelli D, Gartner ZJ, Klein OD.

A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling.

Cell Stem Cell. 2023.

Castillo-Azofeifa D§, Wald T§, Reyes EA, Gallagher A, Schanin J, Vlachos S, Lamarche-Vane N, Bomidi C, Blutt S, Estes MK, Nystul T, Klein OD.

Atoh1+ secretory progenitors possess renewal capacity independent of Lgr5+ cells during colonic regeneration

EMBO J.. 2019.

Castillo-Azofeifa D§, Fazio EN§, Nattiv R§, Good HJ, Wald T, Pest MA, de Sauvage FJ, Klein OD, Asfaha S.

Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.

Development. 2014.

Castillo D§, Seidel K§, Salcedo E, Ahn C, de Sauvage FJ, Klein OD, Barlow LA.