Michael Laird
Senior Director & Principal Scientist, Process Development
Postdoc Mentor
"We are responsible for developing clinical and commercial manufacturing processes for therapeutic proteins."
15
Years at Genentech
27
Publications
Our laboratories are responsible for developing clinical and commercial manufacturing processes for therapeutic proteins. Major expression platforms include E. coli and Chinese Hamster Ovary (CHO) cells.
Our laboratories are housed within Late Stage Cell Culture as part of the Process Research & Development organization. We are responsible for developing clinical and commercial manufacturing processes for therapeutic proteins. Major expression platforms include E. coli and Chinese Hamster Ovary (CHO) cells. Some of the research activities that take place in our laboratories include but are not limited to:
- E. coli and CHO process development activities including scale-up
- Media development for CHO cell cultures including studies focused on fractionating complex undefined nutrients, identifying their active components and formulating chemically defined replacements
- Proteomic and genomic analyses to enhance our understanding of how the culture environment influences the protein production process
- Investigating the relationships between productivity & product quality attributes for both E. coli and CHO processes
- Studying & optimizing cellular metabolism for both E. coli and CHO processes
- Studying glycosylation in CHO cells
- Studying & optimizing protein folding and assembly in both E. coli and CHO production systems
- Developing high-throughput production systems for both E. coli & CHO cells
- Developing novel automated strategies for nutrient feeding for E. coli cultures at small- & large-scale
- Using computational fluid dynamics to understand how equipment differences influence mixing and mass transfer and using this information to optimize mixing conditions and control strategies
Featured Publication
Mechanism of antibody reduction in cell culture production processes.
Biotechnol Bioeng. 2010 Jun 29.
Kao YH, Hewitt DP, Trexler-Schmidt M, Laird MW.
Publications
Air sparging for prevention of antibody disulfide bond reduction in harvested CHO cell culture fluid
Biotechnology and Bioengineering, ISSN: 10970290
Melissa Mun; Stefanie Khoo; Aline Do Minh; James Dvornicky; Melody Schmidt; Yung-Hsiang Kao; Mike Laird
Biotechnology Progress, ISSN: 15206033
Karthik Veeravalli; Mike Laird; Mark Fedesco; Yu Zhang; Christopher Yu (PAC)
Bioengineered, ISSN: 21655987
Karthik Veeravalli; Mike Laird
Eliminating tyrosine sequence variants in CHO cell lines producing recombinant monoclonal antibodies
Biotechnology and Bioengineering, ISSN: 00063592
Lauren Brown; Veronica Carvalhal; Christopher Yu (PAC); Betty Chan; David Michels; Jennifer Wang; Amy Shen; Jan Ressl; Brendon Dusel; Mike Laird
Pharmaceutical Biotechnology: Fundamentals and Applications, Fourth Edition,
Le Dao; Barbara Lippe; Mike Laird
Journal of Biotechnology, ISSN: 01681656
Kristen Douglas; Trissa Borgschulte; Mike Laird
Biotechnology and Bioengineering, ISSN: 00063592
Yung-Hsiang Kao; Dan Hewitt; Melody Schmidt; Mike Laird
Biotechnology Progress, ISSN: 15206033
Lauren Speciner; Erin Mallon; Susan Leung; Mike Laird; Osi Esue
Biotechnology and Bioengineering, ISSN: 00063592
Donglin Guo; Albert Gao; David Michels; Lauren Brown; Marian Eng; Betty Chan; Mike Laird; Boyan Zhang; Christopher Yu (PAC); John Joly; Brad Snedecor; Amy Shen
Protein Expression and Purification, ISSN: 10465928
Shelly Pizarro; Jane Gunson; Matt Field; Rachel Dinges; Stefanie Khoo; Milind Dalal; M Violet Lee; Kimberly Kaleas; Kathryn Moiseff Taylor; Susan Garnick; Dorothea Reilly; Mike Laird; Charles Schmelzer
Molecular Biotechnology, ISSN: 10736085
Brian Horvath; Melissa Mun; Mike Laird
Biotechnology Progress, ISSN: 87567938
Ekta Mahajan; Tim Matthews; Ryan Hamilton; Mike Laird
Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing
Biotechnology and Bioengineering, ISSN: 00063592
Melody Schmidt; Sandy Sargis; Jason Chiu; Stefanie Sze-Khoo; Melissa Mun; Yung-Hsiang Kao; Mike Laird
AIChE Annual Meeting, Conference Proceedings,
Jane Gunson; Mike Laird
Biochemistry, ISSN: 00062960
Katherine Y. Blain; Witek Kwiatkowski; Qinghai Zhao; David La Fleur; Chethana Naik; Tae-Wook Chun; Tatiana Tsareva; Palanisamy Kanakaraj; Mike Laird; Rutul Shah; Lisa George; Indra Sanyal; Paul A. Moore; Borries Demeler; Senyon Choe
Critical Care Medicine, ISSN: 00903493
Kevin Sherer; Yan Li; Xizhong Cui; Xuemei Li; Mani Subramanian; Mike Laird; Mahtab Moayeri; Stephen H. Leppla; Yvonne Fitz; Junwu Su; Peter Q. Eichacker
Molecular Microbiology, ISSN: 0950382X
Tracy L. Raivio; Mike Laird; John Joly; Thomas J. Silhavy
Molecular Microbiology, ISSN: 0950382X
Andrew Kloser; Mike Laird; Ming Deng; Rajeev Misra
Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells
Biotechnology Progress, ISSN: 87567938
Zhilan Hu; Danming Tang; Shahram Misaghi; Guoying Jiang; Christopher Yu (PAC); Mandy Yim; David (ESCC) Shaw; Brad Snedecor; Mike Laird; Amy Shen
Biotechnology Progress, ISSN: 15206033
Zhilan Hu; Wendy Hsu; Abby Pynn; Domingos Ng; Donna Quicho; Yilma Adem; Zephie Kwong; Jacob (Brad) Mauger; John Joly; Brad Snedecor; Mike Laird; Dana Andersen; Amy Shen
Biotechnology Progress, ISSN: 15206033 87567938
Yizhou Zhou; David (ESCC) Shaw; Cynthia Lam; Joni Tsukuda; Mandy Yim; Danming Tang; Salina Louie; Mike Laird; Brad Snedecor; Shahram Misaghi
Biotechnology Progress, ISSN: 15206033 87567938
David (ESCC) Shaw; Mandy Yim; Joni Tsukuda; John Joly; Andy Lin; Brad Snedecor; Mike Laird; Steven E. Lang
Journal of Pharmaceutical Sciences, ISSN: 15206017
Xuan Gao; Andrea Ji; Karthik Veeravalli; Yong Wang; Yonghua (Taylor) Zhang; William Mcgreevy; Kai Zheng; Robert Kelley; Michael W. Laird; Jun Liu; Mary Cromwell
Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality
Biotechnology Progress, ISSN: 87567938
Peggy Ko; Shahram Misaghi; Zhilan Hu; Dejin Zhan; Joni Tsukuda; Mandy Yim; Mark Sanford; David (ESCC) Shaw; Masaru Shiratori; Brad Snedecor; Mike Laird; Amy Shen
Biotechnology Progress, ISSN: 15206033 87567938
Karthik Veeravalli; Tony Schindler; Emily Dong; Masaki Yamada; Ryan Hamilton; Mike Laird
PDA Journal of Pharmaceutical Science and Technology, ISSN: 19482124 10797440
Kyle Zingaro; David (ESCC) Shaw; Jerry Carson; Anke Mayer-Bartschmid; Christian Bender; Christina Alves; Duncan McVey; Nan-Xin Qian; Qingxiang Wei; Mike Laird; Yuan Zhu; Robyn Emmins; John A. Follit; Alison Porter; Andrew J. Racher; Sarah Milne; Ivan Carubelli; Zhimei; Ankita Khatri; Marilyne Failly; Hervé Broly; Frank Lee; Matthew Reeser; Jared Spidel; Karin Anderson; Christine Demaria; Jennifer Di-Carlo; John Gill; Amie Lundquist; Sampath R. Kumar; Tony Gill
Biotechnology Journal, ISSN: 18607314 18606768
Danming Tang; Jayashree Subramanian; Benjamin Haley; Jordan Baker; Lucas Luo; Wendy Hsu; Peter Liu; Wendy Sandoval; Mike Laird; Brad Snedecor; Masaru Shiratori; Shahram Misaghi
Education
- Genentech, Inc., Postdoctoral Fellow – 1999
- Arizona State University, Microbiology, Ph.D. – 1996
- Iowa State University , Biology, B.S. – 1991
